Use of autologous fibrin glue (platelet-poor plasma) in abdominal dermolipectomies.

BACKGROUND: Autologous plasma is endowed with properties that speed up healing, hemostasis, and adhesiveness, in addition to growth factors. Through an established protocol, it was possible to isolate thrombin, as well as the platelet-rich plasma (PRP) and platelet-poor plasma (PPP) fractions. The purpose of this study was to analyze autologous use of thrombin and PPP to foster adhesion between an abdominal dermoadipose flap and the aponeurotic surface in abdominal dermolipectomies. METHODS: The data from 40 patients who underwent abdominal dermolipectomies were analyzed, with 20 patients using thrombin and autologous PPP (Plasma group) and 20 patients with no intervention (Control group). An attempt was made to assess adhesive power by quantifying the serohematic liquid volume gauged during the postoperative days (POD), and also noting the incidence of seroma. Other variables such as age and body mass index (BMI) were also analyzed. RESULTS: The reduction in the aspiration drain debit was statistically relevant only on the first POD in the Plasma group. There was no reduction in the incidence of seroma in these patients. Similarly, age and BMI did not influence these outcomes. CONCLUSION: The PPP fostered adhesion between the abdominal dermoadipose flap and the aponeurotic surface only on the first POD and had no influence whatsoever on the incidence of seroma. There are few reports on the use of PPP for plastic surgery, particularly the autologous type, opening up possibilities for further research projects to expand its use. LEVEL OF EVIDENCE III: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the table of contents or the online instructions to authors www.springer.com/00266.

Protocol for obtaining platelet-rich plasma (PRP), platelet-poor plasma (PPP), and thrombin for autologous use.

BACKGROUND: Plasma has been widely studied and used in many different situations to speed up healing with better tissue adherence and hemostasis. Research projects are now attempting to isolate platelet-rich plasma (PRP) and platelet-poor plasma (PPP), making better use of their properties, particularly during operations and for wounds that are slow to heal. In view of the wide diversity of industrial machines and extraction protocols, together with the variety of industrially produced biologic glues, this article suggests an option for obtaining PRP, PPP, and human thrombin for autologous use. METHODS: A way of obtaining PRP, PPP, and thrombin is reproduced through a protocol defined and established by the authors. Autologous thrombin and plasma were obtained through the collection and successive centrifugation of ten whole blood samples, until the desired hemocomponents were isolated, followed by quantitative and qualitative analyses of the elements obtained. RESULTS: The mean platelet concentration obtained was 6.03 × 10(8) platelets/ml, with a mean thrombin concentration of 33.54 nM, both values compatible with reports in the literature when different protocols are applied. CONCLUSIONS: The protocol described is a good option for the preparation and application of PRP, PPP, and autologous thrombin, particularly as they can be obtained simultaneously, eliminating the possibilities of viral contamination and allergic reactions. Moreover, the cost of this procedure is low, it is easy to perform, and replicable. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article.

Use en autologous plasma in abdominoplasty: previous note.

Autologous plasma began to be studied in the 90's, mainly because its adhesive and angiogenic properties and the presence of growth factors of platelet origin. In fact, plasma can be isolated from autologous manner, from the patient's own blood and obtained in its two parts: a high concentration of platelets (platelet-rich plasma - PRP) and one with low concentration of platelets (platelet-poor plasma - PPP). The present study is in development at the Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro (UFRJ-HUCFF) and Marcilio Dias Naval Hospital (HNMD), both in Rio de Janeiro. The objective is to evaluate the properties of platelet-poor plasma, particularly its adhesive action, in patients referred for restorative abdominal dermolipectomy, to reduce postoperative collections, such as hematoma and seroma, two major complications in this type of surgery.

Uso de plasma autólogo em dermolipectomia abdominal: nota prévia / Use en autologous plasma in abdominoplasty: previous note

O plasma autólogo começou a ser estudado na década de 90, principalmente por suas propriedades adesivas, de angiogênese e pela presença de fatores de crescimento de origem plaquetária. Na verdade, o plasma pode ser isolado de modo autólogo, a partir do sangue do próprio paciente e obtido nas suas duas porções: uma com alta concentração de plaquetas (plasma rico em plaquetas- PRP) e outra com concentração baixa de plaquetas (plasma pobre em plaquetas- PPP). O presente estudo está em desenvolvimento no Hospital Universitário Clementino Fraga Filho, da Universidade Federal do Rio de Janeiro (HUCFF-UFRJ) e no Hospital Naval Marcílio Dias (HNMD), ambos no Rio de Janeiro. O objetivo é avaliar as propriedades do plasma pobre em plaquetas, principalmente a sua ação adesiva, em pacientes com indicação de dermolipectomia abdominal reparadora, de modo a reduzir as coleções no pós operatório, como hematoma e seroma, duas das principais complicações nesse tipo de cirurgia.

Autologous plasma began to be studied in the 90's, mainly because its adhesive and angiogenic properties and the presence of growth factors of platelet origin. In fact, plasma can be isolated from autologous manner, from the patient's own blood and obtained in its two parts: a high concentration of platelets (platelet-rich plasma - PRP) and one with low concentration of platelets (platelet-poor plasma - PPP). The present study is in development at the Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro (UFRJ-HUCFF) and Marcilio Dias Naval Hospital (HNMD), both in Rio de Janeiro. The objective is to evaluate the properties of platelet-poor plasma, particularly its adhesive action, in patients referred for restorative abdominal dermolipectomy, to reduce postoperative collections, such as hematoma and seroma, two major complications in this type of surgery.

Desbridamento e reconstrução cirurgica do tronco após trauma / Desbridament and surgical reconstruction of trunk after trauma

Introdução: A primeira descrição de infecção com gangrena dos tecidos moles foi realizada por Meleney, em 1924. Observou-se que esta doença é de caráter fulminante, com chance de sobrevida quando prontamente diagnosticadas e desbridadas. Objetivo: Sistematizar o tratamento de um menino vítima de trauma com infecção e gangrena. Relato de caso: Menor traumatizado em acidente ciclístico. Apresentava colostomia em alça e fixação externa de bacia, evoluindo com gangrena dos tecidos moles. Foi submetido a desbridamento efetivo de região inguinal, glúteos e dorso, com extensa amputação dos tecidos moles e músculos e reconstrução em 2º tempo. Conclusão: Os autores recomendam que o diagnóstico e o tratamento sejam imediatos para existir uma chance de sobreviver à doença.

Introduction: Gangrene was first description by Meleney, in 1924. This disease has terrible evolution, which can be obstructed with recent diagnostic and debridement. Objective: Systematize the treatment of a child victim of trauma with infection and gangrene. Case report: A patient victim of a cyclist accident, showing colostomy and external pelvis establish, 5 days after surgery was submitted to effective debridement of inguinal region, gluteus maximus muscle and back trunk, extensive amputation and surgical reconstruction in second time. Conclusion: The authors recommend early diagnosis and quickly treatment to patient has any chance of survive.